NGHIÊN CỨU ỨNG DỤNG ENZYME TRONG SẢN XUẤT LECITHIN GIÀU PHOSPHATIDYCHOLINE TỪ DẦU ĐẬU TƯƠNG

Abstract

Commercial lecithin normally contains high phosphatidylcholine content and could be originated from plants, animals or microorganisms. However, soybean is the main material source for lecithin production at industrial scale. In this research, raw lecithin obtained from the dehydration of soybean oil will be utilized to be converted into phosphatidylcholine. The phosphatidylcholinization by commercial enzyme namely phospholipase D from Streptomyces chromofucus (Sigma) was done at conditions as phospholipase D concentration of 0.2U/ml; added choline chloride of 2.5%; pH 5.5-6.5; reaction time of 40 minutes. Converted PC content reached 23.8% from the initial PC content of 11.5%. The research result showed that phospholipase D enzyme could be utilized for the production of phosphatidylcholine enriched lecithin from available soybean source in Vietnam.