NGHIÊN CỨU ẢNH HƯỞNG CỦA MỘT SỐ THÀNH PHẦN MÔI TRƯỜNG ĐẾN KHẢ NĂNG SINH TỔNG HỢP CANTHAXANTHIN CỦA VI KHUẨN Paracoccus carotinifaciens VTP20181

Abstract

The biosynthesis method using bacterial is a practical and economic approach that is being expanded by many scientists and businesses to meet the market's demand for canthaxanthin. The aim of this study is to investigate the influence of some medium components on the ability in canthaxanthin biosynthesis of P. carotinifaciens VTP20181 which was isolated and selected by Food Industries Research Institute. Tested medium components were a number of carbon-containing substrates, nitrogen- containing substances, minerals, vitamins and amino acids. When using sucrose as a carbohydrate substrate, the sample helps P. carotinifaciens VTP20181 to increase the yield of canthaxanthin biosynthesis up to 10.89 mg Cx/l, higher than that of the
control (10.63 mg Cx/l). The sample using nitrogen substrates such as NH4SO4, yeast powder, and yeast extract have canthaxanthin yield which increase by 15%, 43.3%, and 47.7% comparing with the control, respectively. The canthaxanthin yield of P. carotinifaciens VTP20181 in the sample which was added with biotin/glutamate/malate component was improved with the value of 12.91 mgCx/l, 23.66 mgCx/l, and 16.96 mgCx/l, respectively. The results of this research are an important premise for screening and optimizing the composition of the medium for canthaxanthin biosynthesis by P. carotinifaciens VTP20181 at industrial scale.