NGHIÊN CỨU ĐIỀU KIỆN THÍCH HỢP CHO QUÁ TRÌNH CHUYỂN HÓA VÀ TỔNG HỢP TREHALOSE TỪ TINH BỘT SẮN BẰNG PHƯƠNG PHÁP ENZYME

Abstract

The method of producing trehalose from cassava starch using maltooligosyl trehalose synthase (MTSase) and maltooligosyl trehalose trehalohydrolase (MTHase) was highly effective
and could be applied in large-scale production. This research used MTSase and MTHase
enzymes obtained from Saccharolobus solfataricus DSM 1616 strain to convert and synthesize trehalose from cassava starch. Cassava starch solution containing maltooligosaccharide
was hydrolyzed under appropriate conditions: optimum pH of 6.0 and temperature of 55oC;
MTSase-MTHase at 300U / g starch; adding CGTase after 8 hours of reaction, time of metabolism 24 hours. To improve the conversion efficiency of starch into trehalose, hydrolytic
enzyme as isoamylase (1000U / g starch) and CGTase enzyme (20U / g starch) were added.
The results showed that, after the hydrolysis and conversion of cassava starch by enzyme
method, the content of trehalose reached 86.2% of the total sugar. In conclusion, maltooligosyl trehalose synthase and maltooligosyl trehalose trehalohydrolase could be used to produce
trehalose from available cassava starch sources in Vietnam.