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Date Published: 28/06/2025
Online Published: 14/06/2025
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DOI: https://doi.org/10.56283/1859-0381/885
Issue
Vol. 21 No. 3 (2025)
Section
ORIGINAL RESEARCH
How to Cite
1.
VO PM, NGUYEN DT, NGUYEN HT, NGUYEN PT. SELECTION OF AMYLASE-PRODUCING MOLDS FROM Com Ruou (SWEET FERMENTED GLUTINOUS RICE) AS A POTENTIAL CANDIDATE FOR ENZYME INDUSTRY. Tạp chí Dinh dưỡng và Thực phẩm. 2025;21(3):45-55. doi:10.56283/1859-0381/885

SELECTION OF AMYLASE-PRODUCING MOLDS FROM Com Ruou (SWEET FERMENTED GLUTINOUS RICE) AS A POTENTIAL CANDIDATE FOR ENZYME INDUSTRY

Phuong Mai VO1, Duy Tan NGUYEN1, Huu Thanh NGUYEN1, Phu Tho NGUYEN1,
1 An Giang University, Vietnam

Main Article Content

Abstract

Aims: This study aims to isolate and evaluate the amylase enzyme biosynthesic capability of fungal strains derived from Com Ruou (Sweet fermented glutinous rice).

Methods: The research involves the initial isolation and screening of fungal strains for their ability to degrade starch through the formation of clear zones around colonies using starch agar and iodine solution. Potential strains were subsequently cultured in a starch liquid medium to assess amylase enzyme activity. The strain with the highest amylase biosynthesis was identified using molecular biology techniques.

Results: A total of 14 fungal strains were isolated and analyzed for morphological characteristics, microscopic observation, and starch degradation capability. Results indicated that the strain CRBK1 exhibited the highest amylase activity, reaching approximately 70% and 3.5 U/mL, significantly surpassing the other strains. ITS rDNA sequencing identified CRBK1 as belonging to the species Rhizopus arrhizus, with over 96% similarity to strains in the GenBank database.

Conclusion: This research highlights the potential for utilizing indigenous fungal sources for amylase enzyme production. Future research will focus on optimizing fermentation processes, improving the purification of amylase from Rhizopus arrhizus, and assessing the enzyme's stability in food industry and bioenergy applications.

Keywords

Com ruou, amylase enzyme, starch degradation, Rhizopus arrhizus

Article Details

References

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