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Date Published: 14/11/2024
Abstract Views: 45
Views PDF: 12
DOI: https://doi.org/10.56283/1859-0381/792
Issue
Vol. 20 No. 6 (2024): HỘI NGHỊ KHOA HỌC VIỆN DINH DƯỠNG NĂM 2024
Section
METHODOLOGY
How to Cite
1.
BUI TKT, BUI TTN, DUONG TL, TRAN QB. OPTIMAL PROTOCOL FOR GENOTYPING THE NPC1L1 RS2070895 POLYMORPHISM USING THE POLYMERASE CHAIN REACTION - RESTRICTION FRAGMENT LENGTH POLYMORPHISM METHOD. Tạp chí Dinh dưỡng và Thực phẩm. 2024;20(6):193-200. doi:10.56283/1859-0381/792

OPTIMAL PROTOCOL FOR GENOTYPING THE NPC1L1 RS2070895 POLYMORPHISM USING THE POLYMERASE CHAIN REACTION - RESTRICTION FRAGMENT LENGTH POLYMORPHISM METHOD

Thi Khanh Thuan BUI1, Thi Thuy Nga BUI2, Tuan Linh DUONG2, Quang Binh TRAN2,
1 Nam Dinh University of Nursing
2 National Institute of Nutrition, Vietnam

Main Article Content

Abstract

Aims: To develop and optimize a method for genotyping the SNP NPC1L1 rs2072183 in the Vietnamese population using polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP).


Methods: Software was used to design primer pairs and molecular biology techniques to optimize primer pairs and appropriate thermal cycling. Sanger sequencing method was used to determine the standard genotype of rs2072183 polymorphism. Experiments were performed on 16 DNA samples extracted from whole blood of Vietnamese people.


Results: A protocol for genotyping the rs2072183 has been developed and optimized using the PCR-RFLP method with accurate results.


Conclusions: The PCR-RFLP protocol for analyzing NPC1L1rs2072183 polymorphism has high accuracy, fast results, low cost and it can be applied on larger sample samples in different populations to conduct research on the association of this polymorphism with dyslipidemia in Vietnamese people.

Article Details

Keywords

rs2072183, NPC1L1gene, Dyslipidemia,, RFLP-PCR

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