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DOI: https://doi.org/10.56283/1859-0381/651
Issue
Vol. 20 No. E (2024)
Section
ORIGINAL RESEARCH
How to Cite
1.
Tống Đức M, Nguyễn VC, NGUYEN VB, NGUYEN TTT, Le TA, HOANG TT, VU TH, NGUYEN TD, BUI KT. INITIAL STEPS OF RESEARCH INDEVELOPING A LATERAL FLOW IMMUNOASSAY KIT FOR RAPID DETECTION OF OCHRATOXIN A IN FOOD. Tạp chí Dinh dưỡng và Thực phẩm. 20(E):19-31. doi:10.56283/1859-0381/651

INITIAL STEPS OF RESEARCH INDEVELOPING A LATERAL FLOW IMMUNOASSAY KIT FOR RAPID DETECTION OF OCHRATOXIN A IN FOOD

Duc Minh TONG1,, Van Chuyen NGUYEN2, Van Ba NGUYEN1, Thi Thu Trang NGUYEN1, Tuan Anh LE1, Thi Truong HOANG1, Thi Hoa VU1, Trong Dat NGUYEN3, Khanh Toan BUI4
1 Vietnam Military Medical University
2 Học viện Quân Y 103
3 Military Insitute of Preventive Medicine, Vietnam
4 Tay Nguyen Institute of Hygiene and Epidemiology, Vietnam

Main Article Content

Abstract

Aims: To determine some technical parameters of rapid test strips to detect Ochratoxin A.


Methods: Research was conducted at the Department of Military Hygiene, Military Medical Academy. We had optimized a number of test parameters including: pre-treatment of conjugate pads, antibody concentration covering the gold nanoparticles, capture antibody concentration, and running buffer. Determine the technical parameters of test strips manufactured to detect Ochratoxin A, including cross-reactivity, stability, sensitivity, specificity.


Results: Antibody concentration of 20 µg/ml covering the gold particle reached saturation state and LOD is 1 µg/ml. The capture antibody concentration was 0.75 mg/ml; Lactose 10% and sucrose 10% enhanced the release of gold particle-antibody complexes through the conjugate membrane. The test strip did not cross-react with standard samples of Aflatoxin B1, Aflatoxin M1, and Patulin at the concentrations tested. 


Conclusion: The results show that optimizing the parameters are important. Evaluation of the technical parameters of the rapid test strip for Ochratoxin A detection shows that the test strip has sensitivity and specificity at a high level.

Article Details

Keywords

Immunochromatographic test strips, quick detection, Ochratoxin A

References

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