XÂY DỰNG PHƯƠNG PHÁP XÁC ĐỊNH HÀM LƯỢNG CURCUMIN TRONG THỰC PHẨM BẢO VỆ SỨC KHỎE BẰNG SẮC KÝ LỎNG HIỆU NĂNG CAO

Abstract

Objective: To develop a method to detect curcumin concentration in health supplement by high performance liquid chromatography with a photodiode array detector.
Method: Curcurmin was extracted by sonication in methanol for 30 min and separated
by a reversed phase C18 column using a mobile phase including buffer, methanol and
acetonitrile. The flow rate was set to 1.0 ml.min-1 and the PDA detector was performed
at 426 nm. Result: The validated method proved to be linear in the range of 0.02 to 5.0
µg.ml-1 with R2 was 0.9999. Detection and quantification limits were 0.26-0.37 µg.g-1
or µg.ml-1 and 0.88-1.22 µg.g-1 or µg.ml-1, respectively. The accuracy of curcumin
was within the range of 75-95%, with the relative standard deviation (RSD%) of 0.4-
8.4%. Conclusion: The validated parameters have met the requirement of Association
of Official Agricultural Chemists (AOAC). This reliable method would be useful for
the study and quality control of curcumin in health supplement.