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Date Published: 31/10/2023
Abstract Views: 137
Views PDF: 84
DOI: https://doi.org/10.56283/1859-0381/496
Issue
Vol. 19 No. 4+5 (2023)
Section
ORIGINAL RESEARCH
How to Cite
1.
Lê TC, LE HD, NGUYEN VS. VALIDATION METHOD FOR DETERMINATION OF 5,6-DEHYDROKAVAIN IN FRESH LEAVES AND LEAF POWDER OF ALPINIA ZERUMBET BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. Tạp chí Dinh dưỡng và Thực phẩm. 2023;19(4+5):39-47. doi:10.56283/1859-0381/496

VALIDATION METHOD FOR DETERMINATION OF 5,6-DEHYDROKAVAIN IN FRESH LEAVES AND LEAF POWDER OF ALPINIA ZERUMBET BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Thi Cuc LE1,, Hong Dzung LE1, Van Sy NGUYEN1
1 National Institute of Nutrition, Hanoi, Viet Nam

Main Article Content

Abstract

Aims: To validate a method to detect 5,6-dehydrokavain concentration in fresh leaves and leaf powder of  Alpinia zerumbet by high performance liquid chromatography with a photodiode array detector.


Methods: 5,6-dehydrokavain was extracted by sonication in methanol at 40 oC, then separated by a reversed phase C8 column using a mobile phase including methanol and  1% aqueous acetic acid. The analysis time is 10 minutes and the PDA detector was performed at 343 nm. Validation parameters were performed according to the guidelines of AOAC 2016.


Results: The validated method proved to be linear in the range of 0.05 to 10 μg/ml (correlation coefficient: r = 1). The limit of detection in fresh leaves and leaf powder of  Alpinia zerumbet were -30.3 µg/100g and 60.6 µg/100g, respectively. The limit of quantification in fresh leaves and leaf powder of  Alpinia zerumbet were 100 µg/100g and 200 µg/100g, respectively. The accuracy was within the range from 99,7 to 104.4 %, with the relative standard deviation (RSD%) of 1.0 – 6.6 %.


Conclusion: The validated parameters have met the requirement of Association of Official Agricultural Chemists (AOAC). This reliable method would be useful for the study and determination of 5,6-dehydrokavain in fresh leaves and leaf powder of  Alpinia zerumbet.

Article Details

Keywords

5,6-dehydrokavain, shell ginger, 𝘈𝘭𝘱𝘪𝘯𝘪𝘢 𝘻𝘦𝘻𝘶𝘮𝘣𝘦𝘵, high performance liquid chromatography

References

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