XÁC ĐỊNH HOẠT TÍNH ỨC CHẾ ENZYME α-amylase VÀ α-glucosidase CỦA CAO CHIẾT NƯỚC CỎ SỮA LÁ LỚN (Euphorbia hirta L.)

Abstract

Euphorbia hirta L. (Cỏ sữa lá lớn – CSLL) is a herbal medicine that could reduce the glycemic index. It is used in the prevention and treatment of diabetes thanks to its ability to inhibit
the activity of two starch hydrolyzate enzymes in human body, which are α-amylase and
α-glucosidase. The inhibitory activity of inhibiting enzymes (α-amylase and α-glucosidase)
in vitro of CSLL extract was determined by UV-VIS molecular absorption spectroscopy. The
results of the study showed that extract of Euphorbia hirta L. was able to inhibit α-amylase
enzyme (IC50 = 967 µg / mL) and inhibit α-glucosidase enzyme (IC50 = 53.96 µg / mL). At
the same time, the study results also showed that inhibition of α-amylase and α-glucosidase
enzymes was correlated with total Flavonoid content in extract of Euphorbia hirta L., with
IC50 value of 17.6 and 0.982 µg / Ml, respectively.