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OPTIMIZATION OF ANALYSIS OF LPL RS320 POLYMORPHISM ASSOCIATED WITH DYSLIPIDEMIA USING THE ALEN-SPECIFIC PRIMER METHOD
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Abstract
Aims: To develop a genotyping process of the LPL rs320 single nucleotide polymorphism (SNP LPL rs320) using the AS-PCR method on DNA samples extracted from blood of adults aged 40-64 years.
Methods: Using the AS-PCR method to analyze the genotype of the SNP LPL rs320 on 80 DNA samples of Vietnamese people. Using software to design allele-specific primers and molecular biology techniques to optimize primer pairs and appropriate thermal cycles. Sanger sequencing method is used to determine the standard genotype of this single nucleotide polymorphism.
Results: The genotyping procedure, including primer pairs, reaction components, and thermal cycling for gene amplification of the SNP LPL rs320 using AS-PCR, was developed and optimized on adult human DNA samples.
Conclusion: The AS-PCR procedure for analyzing SNP LPL rs320 has high accuracy, fast results and can be repeated on larger sample populations, in different populations to conduct research on the association of this polymorphism with dyslipidemia in Vietnamese population.
Keywords
AS-PCR, genotyping, LPL gene, rs320, dyslipidemia.
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References
2. Hoàng Văn Minh. Điều tra quốc gia các yếu tố nguy cơ bệnh không lây nhiễm tại Việt Nam. 2021.
3. Teslovich TM, Musunuru K, Smith AV, Edmondson AC, Stylianou IM, Koseki M, et al. Biological, clinical and population relevance of 95 loci for blood lipids. Nature. 2010 Aug;466(7307):707–13. doi: 10.1038/nature09270.
4. Daoud MS, Ataya FS, Fouad D, Alhazzani A, Shehata AI, Al-Jafari AA. Associations of three lipoprotein lipase gene polymorphisms, lipid profiles and coronary artery disease. Biomed Rep. 2013;1(4):573-82. doi:10.3892/br.2013.126
5. rs320 (SNP) - Population genetics - Homo_sapiens - Ensembl genome browser 113. Accessed October 21, 2024. Available from https://asia.ensembl.org/Homo_sapiens/Variation/
6. Wangkumhang P, Chaichoompu K, Ngamphiw C, Ruangrit U, Chanprasert J, et al. WASP: a Web-based Allele-Specific PCR assay designing tool for detecting SNPs and mutations. BMC Genomics. 2007 Aug 14;8:275. doi:10.1186/1471-2164-8-275.
7. SNP - NCBI [Internet]. Nih.gov. 2026 [cited 2026 Jun 1]. Available from: https://www.ncbi.nlm.nih.gov/snp/?term=rs320
8. Primer3 Input [Internet]. primer3.ut.ee. Accessed June 1, 2026. Available from: https://primer3.ut.ee/
9. Ahmed Z, Zeeshan S, Mendhe D, Dong X. Human gene and disease associations for clinical‐genomics and precision medicine research. Clin Transl Med. 2020 jan;10(1):297-318. doi: 10.1002/ctm2.28.
10. Santamarina-Fojo S, Dugi KA. Structure, function and role of lipoprotein lipase in lipoprotein metabolism. Curr Opin Lipidol. 1994 Apr;5(2):117-25. doi:10.1097/00041433-199404000-00008.
11. Goodarzi MO, Guo X, Taylor KD, Quinones MJ, Saad MF, Yang H, et al. Lipoprotein Lipase Is a Gene for Insulin Resistance in Mexican Americans. Diabetes. 2003 Dec 23;53(1):214–20. 10.2337/diabetes.53.1.214
12. Barcellini-Couget S, Pradines-Figuères A, Roux P, Dani C, Ailhaud G. The regulation by growth hormone of lipoprotein lipase gene expression is mediated by c-fos protooncogene. Endocrinology. 1993 Jan;132(1):53-60. doi:10.1210/endo.132.1.8419145.
13. Ezzat, El Abd Need, Mahmoud Ema, El Sheimy Ha. Study of Lipoprotein Lipase gene variants in dyslipidemic type-2 diabetes mellitus. Egypt J Hosp Med. 2023;90(1):1102-8.
14. Darawi MN, Ai-Vyrn C, Ramasamy K, et al. Allele-specific polymerase chain reaction for the detection of Alzheimer’s disease-related single nucleotide polymorphisms. BMC Med Genet. 2013;14(1):27.
15. Tun KLP, Latt AZ, Naing WPP, Htwe SS, Ko YK, Mar WW, et al. Identification of JAK2 (V617F) mutation in myeloproliferative neoplasms by using Allele Specific Polymerase Chain Reaction (AS-PCR). Am J Mol Biol. 2020;10(4):4. 10.4236/ajmb.2020.104019
16. Mai Phương Thảo, Lê Thị Kim Hoàng. Xây dựng quy trình AS-PCR xác định điểm đa hình rs2231142 trên gen ABCG2. Y Học Tp Hồ Chí Minh. 15/3/2018;22(1):224-30.
17. Bogari NM, Aljohani A, Dannoun A, Elkhateeb O, Porqueddu M,et al. Association between HindIII (rs320) variant in the lipoprotein lipase gene and the presence of coronary artery disease and stroke among the Saudi population. Saudi J Biol Sci. 2020 Aug;27(8):2018-24. doi: 10.1016/j.sjbs.2020.06.029.
18. Cao L, Li Q, Chen X. The HindIII and PvuII polymorphisms of lipoprotein lipase (LPL) gene reduce the risk of ischemic stroke (IS). Medicine (Baltimore). 2018 May;97(18):e0483. doi: 10.1097/MD.0000000000010483.
19. Phạm Thị Bích Đào, Dương Tuấn Linh, Bùi Thị Thúy Nga, Nguyễn Ánh Ngọc, Trần Quang Thuyên, et al. Tối ưu quy trình phân tích đa hình đơn nucleotide rs320 thuộc gen lipoprotein lipasa ở người Việt Nam. HNUE J Sci. 2020. 65(3):123-9. doi:10.18173/2354-1059.2020-0015.