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Date Published: 28/06/2025
Online Published: 25/06/2025
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DOI: https://doi.org/10.56283/1859-0381/923
Issue
Vol. 21 No. 3 (2025)
Section
METHODOLOGY
How to Cite
1.
BUI TTN, PHUNG THG, NGUYEN AN, TRAN QB. DEVELOPMENT OF AN ALLEN-SPECIFIC PCR METHOD FOR DETECTING SH2B1 RS7498665 POLYMORPHISM ASSOCIATED WITH ADULT OVERWEIGHT AND OBESITY. Tạp chí Dinh dưỡng và Thực phẩm. 2025;21(3):165-174. doi:10.56283/1859-0381/923

DEVELOPMENT OF AN ALLEN-SPECIFIC PCR METHOD FOR DETECTING SH2B1 RS7498665 POLYMORPHISM ASSOCIATED WITH ADULT OVERWEIGHT AND OBESITY

Thi Thuy Nga BUI1, Thi Huong Giang PHUNG2, Anh Ngoc NGUYEN1, Quang Binh TRAN1,
1 National Institute of Nutrition, Vietnam
2 Thanh Dong University, Vietnam

Main Article Content

Abstract

Aims: To develop a genotyping protocol for single nucleotide polymorphism (SNP) rs7498665 on SH2B1 gene using allele-specific primer method (AS-PCR) on total DNA samples of adult people.

Methods: Specific primers for the AS-PCR method were designed using Primer3 bioinformatics software and PCR reaction conditions were optimized at different annealing temperatures. The reliability of the AS-PCR method was confirmed by Sanger sequencing.

Results: A protocol for geneotyping the rs7498665 has been developed and optimized using the AS-PCR method with accurate results.

Conclusion: The AS-PCR protocol for genotyping SH2B1 rs7498665 is highly accurate, provides rapid results and can be performed with larger sample sizes as well as on different populations to study the association of this polymorphism with overweight and obesity in Vietnamese populations.

Keywords

SNP rs7498665, SH2B1 gene, AS-PCR, overweight, obesity, genotyping.

Article Details

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