VALIDATION OF METHOD FOR DETERMINATION OF 4-HYDROXYDERRICIN VÀ XANTHOANGELOL IN FUNCTION FOOD BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Main Article Content
Abstract
Aims: To validate a method to determine of 4-hydroxyderricin và xanthoangelol concentration in functional food by high performance liquid chromatography with a photodiode array detector.
Methods: 4-hydroxyderricin and xanthoangelol were extracted by sonication in methanol 80% in water for 30 min and separated by a reversed phase C18 column using a mobile phase including methanol and water. The flow rate was set to 1.0 ml.min-1 and the PDA detector was performed at 370 nm. \
Results: The validated method proved to be linear in the range of 4.7 – 235 µg.ml-1 for 4-hydroxyderricin and 5.35 – 267.5 µg.ml-1 for xanthoangelol with R was 0.9999, respectively. The quantification limit of 4-hydroxyderricin and xanthoangelol were 0.24 mg/g and 0.35 mg/g, respectively. The recovery of 4-hydroxyderricin và xanthoangelol was within the range of 95.7 – 100.7%, with the coefficient of variation (CV%) of 2.9 - 4.6%.
Conclusion: The validated parameters have met the requirement of Association of Official Analytical Collaboration (AOAC). This reliable method would be useful for the study and quality control of 4-hydroxyderricin và xanthoangelol in functional food.
Article Details
Keywords
4-hydroxyderricin và xanthoangelol, thực phẩm chức năng, sắc ký lỏng hiệu năng cao
References
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