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OPTIMAL PROTOCOL FOR GENOTYPING THE LIPC RS2070895 POLYMORPHISM USING THE ALLELE-SPECIFIC PRIMER METHOD
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Abstract
Aims: To develop a protocol for geneotyping the LIPC rs2070895 single nucleotide polymorphism (SNP), using the allele-specific primer (AS-PCR) method.
Methods: Allele-specific primers were designed and molecular biology techniques were used to optimize primer pairs and appropriate thermal cycles. Sanger sequencing method was used to determine the standard genotypes of the SNP. The experiments were applied on 16 DNA samples of Vietnamese people.
Results: A protocol for geneotyping the rs2070895 has been developed and optimized using the AS-PCR method with accurate results.
Conclusion: The AS-PCR protocol for analyzing rs2070895 polymorphism has high accuracy, fast results, low cost and it can be applied on larger sample samples in different populations to conduct research on the association of this polymorphism with dyslipidemia in Vietnamese people.
Article Details
Keywords
AS-PCR, LIPC gene, rs2070895, dyslipidemia
References
2. Báo động thực trạng thừa cholesterol: Hệ lụy và giải pháp - Tin liên quan - Cổng thông tin Bộ Y tế. Accessed September 22, 2024. https://moh.gov.vn
3. Bộ Y tế. Hướng dẫn chẩn đoán và điều trị bệnh rối loạn chuyển hóa, nội tiết. Published online 2015.
4. L. N, Chandran Pillai ALP. Dyslipidemia: Genetics and Role in the Metabolic Syndrome. In: Kelishadi R, ed. Dyslipidemia - From Prevention to Treatment. InTech; 2012. doi:10.5772/28188
5. Opap K, Mulder N. Recent advances in predicting gene–disease associations. F1000Res. 2017;6:578. doi:10.12688/f1000research.10788.1
6. Antamarina-Fojo S, Dugi KA. Structure, function and role of lipoprotein lipase in lipoprotein metabolism: Current Opinion in Lipidology. 1994;5(2):117-125.
7. Kobayashi J, Miyashita K, Nakajima K, Mabuchi H. Hepatic Lipase: a Comprehensive View of its Role on Plasma Lipid and Lipoprotein Metabolism. J Atheroscler Thromb. 2015;22(10):1001-1011. doi:10.5551/jat.31617
8. Wei W, Hu T, Luo H, et al. The cross‐sectional study of hepatic lipase SNPs and plasma lipid levels. Food Science & Nutrition. 2020;8(2):1162-1172.
9. Verma P. The rs2070895 (-250G/A) Single Nucleotide Polymorphism in Hepatic Lipase (HL) Gene and the Risk of Coronary Artery Disease in North Indian Population: A Case-Control Study. Journal of Clinical and Diagnosis Research. 2016;10. doi:10.7860/JCDR/2016/20496.8378
10. Zhao S, Xie X, Nie S. The −250G→A polymorphism in the human hepatic lipase gene promoter affects blood lipids in Chinese. Clinica Chimica Acta. 2006;365(1-2):149-152. doi:10.1016/j.cca.2005.08.013
11. De Andrade F, Silveira F, Arsand M, et al. Association between −250G/A polymorphism of the hepatic lipase gene promoter and coronary artery disease and HDL‐C levels in a Southern Brazilian population. Clinical Genetics. 2004;65(5):390-395. doi:10.1111/j.0009-9163.2004.00243.x
12. Wangkumhang P, Chaichoompu K, Ngamphiw C, et al. WASP: a Web-based Allele-Specific PCR assay designing tool for detecting SNPs and mutations. BMC Genomics. 2007;8(1):275. doi:10.1186/1471-2164-8-275
13. Ahmed Z, Zeeshan S, Mendhe D, Dong X. Human gene and disease associations for clinical‐genomics and precision medicine research. Clinical & Translational Med. 2020;10(1):297-318. doi:10.1002/ctm2.28
14. Meng L, Ruixing Y, Yiyang L, et al. Association of LIPC -250G>A polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations. Lipids Health Dis. 2010;9(1):28. doi: 10.1186/1476-511X-9-28.