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ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES FROM LEAF EXTRACTS OF Cymbopogon citratus
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Abstract
Aims: The study aimed to determine the active ingredients, antibacterial and antioxidant properties of C. citratus leaf extract in water, 96% ethanol, and 96% methanol.
Methods: The qualitative method for the identification of natural compounds in C. citratus leaf extract based on color reactions. The antioxidant capacity of the extract was investigated using the diphenylpicrylhydrazyl (DPPH) free radical scavenging method. The antibacterial capacity was investigated using the agar-well diffusion method.
Results: Active ingredients found in C. citratus leaf extract included alkaloids, flavonoids, saponins, terpenoids, coumarin quinone, and tannins. The greatest results for C. citratus leaf extract were obtained with a 96% methanol solvent.. The antibacterial activity of the extract on E. coli was 2.50 ± 0.50 and the highest antioxidant efficiency was 95.77% with an IC50 value of 298.40 μg/mL in C. citratus leaf extract in solvent methanol 96%.
Conclusion: Extracts from 96% methanol solvent had the ability to extract most of the compounds in C. citratus leaf extract such as: alkaloids, flavonoids, saponins, terpenoids, quinones, and tannins. All 3 types of C. citratus leaf extracts in water, 96% ethanol, and 96% methanol had antibacterial properties. From the above results, it can be seen that C. citratus leaves are a potential material for studies on antibacterial, antioxidant properties and other studies on natural compounds with biological activity.
Article Details
Keywords
leaf extract, antibacterial, antioxidant, 𝘊𝘺𝘮𝘣𝘰𝘱𝘰𝘨𝘰𝘯 𝘤𝘪𝘵𝘳𝘢𝘵𝘶𝘴
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